A STUDY OF THE FRAGMENTATION OF SINGLY AND MULTIPLY CHARGED IONS PRODUCED BY 252Cf FISSION FRAGMENT BOMBARDMENT OF POLYPEPTIDES BOUND TO NITROCELLULOSE
نویسنده
چکیده
A time-of-flight mass spectrometric study of the prompt and metastable decomposition of ions produced by “‘Cf fission fragment bombardment is presented. Results for a series of polypeptides with molecular weights between 1000 and 14000 u are given. Comparisons are made between the amount of fragmentation observed in ions desorbed from bulk (electrosprayed) polypeptide samples and the amount of fragmentation observed in the corresponding ions desorbed from polypeptides bound to nitrocellulose. Ions desorbed from nitrocellulose were found to undergo much less decomposition than those desorbed from bulk polypeptide. Details of the slow unimolecular decay of high molecular weight multiply protonated polypeptides were investigated for the first time. The metastable decomposition of multiply protonated polypeptides was found to occur, in substantial part, with the formation of two charged fragmentation products.
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تاریخ انتشار 2001